Vaccination has transformed the landscape of infectious disease prevention, positively impacting human health outcomes. However, vaccine confidence has become fragmented, influenced by misinformation, political polarization, and historical healthcare disparities. This brief highlights common workplace barriers to vaccination and offers proven strategies to increase confidence and uptake—especially among high-risk populations. By improving access, strengthening infrastructure, and building trust, employers can support employee health, counter misinformation, and reduce preventable healthcare costs.